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bioRxiv · 10.1101/316125

PySight: plug and play photon counting for fast intravital microscopy

Har-Gil, H.·Golgher, L.·Israel, S.·Kain, D.·Cheshnovksy, O.·Parnas, M.·Blinder, P.
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Abstract

Imaging increasingly large neuronal populations at high rates pushed multi-photon microscopy into the photon-deprived regime. We present PySight, an add-on hardware and software solution tailored for photon-deprived imaging conditions. PySight more than triples the median amplitude of neuronal calcium transients in awake mice, and facilitates single-trial intravital voltage imaging in fruit flies. Its unique data streaming architecture allowed us to image a fruit flys olfactory response over 234 x; 600 x; 330{micro}m3 at 73 volumes per second, outperforming top-tier imaging setups while retaining over 200 times lower data rates. PySight requires no electronics expertise nor custom synchronization boards, and its open-source software is extensible to any imaging method based on single-pixel (bucket) detectors. PySight offers an optimal data acquisition scheme for ever increasing imaging volumes of turbid living tissue.

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Har-Gil, H., Golgher, L., Israel, S., Kain, D., Cheshnovksy, O., Parnas, M., Blinder, P.. 2018-05-09. PySight: plug and play photon counting for fast intravital microscopy. https://doi.org/10.1101/316125

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