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bioRxiv · 10.1101/310102

Investigating the effect of Target of Rapamycin kinase inhibition on the Chlamydomonas reinhardtii phosphoproteome: from known homologs to new targets

Werth, E. G.·McConnell, E. W.·Couso, I.·Perrine, Z.·Crespo, J. L.·Umen, J. G.·Hicks, L. M.
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Abstract

O_LITarget of Rapamycin (TOR) kinase is a conserved regulator of cell growth whose activity is modulated in response to nutrients, energy and stress. Key proteins involved in the pathway are conserved in the model photosynthetic microalga Chlamydomonas reinhardtii, but the substrates of TOR kinase and downstream signaling network have not been elucidated. Our study provides a new resource for investigating the phosphorylation networks governed by the TOR kinase pathway in Chlamydomonas.\nC_LIO_LIWe used quantitative phosphoproteomics to investigate the effects of inhibiting Chlamydomonas TOR kinase on dynamic protein phosphorylation. Wild-type and AZD-insensitive Chlamydomonas strains were treated with TOR-specific chemical inhibitors (rapamycin, AZD8055 and Torin1), after which differentially affected phosphosites were identified.\nC_LIO_LIOur quantitative phosphoproteomic dataset comprised 2,547 unique phosphosites from 1,432 different proteins. Inhibition of TOR kinase caused significant quantitative changes in phosphorylation at 258 phosphosites, from 219 unique phosphopeptides.\nC_LIO_LIOur results include Chlamydomonas homologs of TOR signaling-related proteins, including a site on RPS6 with a decrease in phosphorylation. Additionally, phosphosites on proteins involved in translation and carotenoid biosynthesis were identified. Follow-up experiments guided by these phosphoproteomic findings in lycopene beta/epsilon cyclase showed that carotenoid levels are affected by TORC1 inhibition and carotenoid production is under TOR control in algae.\nC_LI

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Werth, E. G., McConnell, E. W., Couso, I., Perrine, Z., Crespo, J. L., Umen, J. G., Hicks, L. M.. 2018-04-28. Investigating the effect of Target of Rapamycin kinase inhibition on the Chlamydomonas reinhardtii phosphoproteome: from known homologs to new targets. https://doi.org/10.1101/310102

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