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bioRxiv · 10.1101/310094

Xpert Ultra can unambiguously identify specific rifampicin resistance-conferring mutations

Ng, K. C. S.·van Deun, A.·Meehan, C. J.·Torrea, G.·Driesen, M.·Gabriels, S.·Rigouts, L.·Andre, E.·de Jong, B. C.
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Abstract

Introduction Introduction Methods Microorganisms Results Conclusions References The deluge of data produced by XpertMTB/RIF (Cepheid) can help improve global rifampicin-resistant tuberculosis (RR-TB) control strategies through molecular epidemiological surveillance (1, 2). Recently, a new version of the test - Xpert Ultra (hereinafter called Ultra) was released (3). Determining the relationship between RR-conferring rpoB mutations, Ultra probes, and melting temperature shifts ({Delta}Tm) - the difference between mutant and wildtype melting temperatures - allows Ultra results to be utilized for rapid detection of RR-TB strains and related underlying rpoB mutations. ...

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Ng, K. C. S., van Deun, A., Meehan, C. J., Torrea, G., Driesen, M., Gabriels, S., Rigouts, L., Andre, E., de Jong, B. C.. 2018-05-09. Xpert Ultra can unambiguously identify specific rifampicin resistance-conferring mutations. https://doi.org/10.1101/310094

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