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bioRxiv · 10.1101/303180

Cdc23/Mcm10 Primase Generates the Lagging Strand-Specific Ribonucleotide Imprint in Fission Yeast

Singh, J.·Singh, B.·Bisht, K. K.·Upadhyay, U.·Kushwaha, A. C.·Nanda, J. S.·Srivastava, S.·Klar, A. J.
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Abstract

The developmental asymmetry of fission yeast daughter cells derives from inheriting \"older Watson\" versus \"older Crick\" DNA strand from the parental cell, strands that are complementary but not identical with each other. A novel DNA strand-specific \"imprint\", installed during DNA replication at the mating-type locus (mat1), imparts competence for cell type inter-conversion to one of the two chromosome replicas. The biochemical nature of the imprint and the mechanism of its installation are still not understood. The catalytic subunit of DNA Polymerase (Pol) has been implicated in the imprinting process. Based on its known biochemical function, Pol might install the mat1 imprint during lagging strand synthesis. The nature of the imprint is not clear: it is either a nick or a ribonucleotide insertion. Our investigations do not support a role of Pol in nicking through putative endonuclease domains but confirm its role in installing an alkali-labile moiety as the imprint. A detailed genetic and molecular analysis reveals a direct role of the Cdc23/Mcm10 primase activity in installing the imprint in cooperation with Pol and Swi1.

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Singh, J., Singh, B., Bisht, K. K., Upadhyay, U., Kushwaha, A. C., Nanda, J. S., Srivastava, S., Klar, A. J.. 2018-04-17. Cdc23/Mcm10 Primase Generates the Lagging Strand-Specific Ribonucleotide Imprint in Fission Yeast. https://doi.org/10.1101/303180

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