An optimized toolkit for precision base editing

Zafra, M. P.; Schatoff, E. M.; Katti, A.; Foronda, M.; Breinig, M.; Schweitzer, A. Y.; Simon, A.; Han, T.; Goswami, S.; Montgomery, E.; Thibado, J.; Sanchez-Rivera, F. J.; Shi, J.; Vakoc, C. R.; Lowe, S. W.; Tschaharganeh, D. F.; Dow, L. E.

doi:10.1101/303131

bioRxiv · 10.1101/303131

An optimized toolkit for precision base editing

Zafra, M. P.·Schatoff, E. M.·Katti, A.·Foronda, M.·Breinig, M.·Schweitzer, A. Y.·Simon, A.·Han, T.·Goswami, S.·Montgomery, E.·Thibado, J.·Sanchez-Rivera, F. J.·Shi, J.·Vakoc, C. R.·Lowe, S. W.·Tschaharganeh, D. F.·Dow, L. E.

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Abstract

CRISPR base editing is a potentially powerful technology that enables the creation of genetic mutations with single base pair resolution. By re-engineering both DNA and protein sequences, we developed a collection of constitutive and inducible base editing vector systems that dramatically improve the ease and efficiency by which single nucleotide variants can be created. This new toolkit is effective in a wide range of model systems, and provides a means for efficient in vivo somatic base editing.

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Zafra, M. P., Schatoff, E. M., Katti, A., Foronda, M., Breinig, M., Schweitzer, A. Y., Simon, A., Han, T., Goswami, S., Montgomery, E., Thibado, J., Sanchez-Rivera, F. J., Shi, J., Vakoc, C. R., Lowe, S. W., Tschaharganeh, D. F., Dow, L. E.. 2018-04-17. An optimized toolkit for precision base editing. https://doi.org/10.1101/303131

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