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bioRxiv · 10.1101/300608

Catalysis of cataract-associated human γD crystallin aggregation via dynamic disulfide exchange

Abstract

Several mutations in human {gamma}D-crystallin (H{gamma}D), a long-lived eye lens protein, cause misfolding and aggregation, leading to cataract. Surprisingly, wild-type H{gamma}D catalyzes aggregation of its cataract related W42Q variant while itself remaining soluble - the inverse of the classical prion-like scenario whereby misfolded polypeptides catalyze aggregation of natively folded ones. The search for a biochemical mechanism of catalysis of W42Q aggregation by WT has revealed that WT H{gamma}D can transfer a disulfide bond to the W42Q variant. The transferred disulfide kinetically traps an aggregation-prone intermediate made accessible by the W42Q mutation, facilitating light-scattering aggregation of the W42Q variant. The aggregating variant thus becomes a disulfide sink, removing the disulfides from solution. Such redox \"hot potato\" competitions among wild-type and mutant or modified polypeptides may be relevant for many long-lived proteins that function in oxidizing environments. In these cases aggregation may be forestalled by inhibiting disulfide flow toward damaged polypeptides.

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BibTeXRIS

Serebryany, E., Yu, S., Trauger, S. A., Budnik, B., Shakhnovich, E. I.. 2018-04-12. Catalysis of cataract-associated human γD crystallin aggregation via dynamic disulfide exchange. https://doi.org/10.1101/300608

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