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bioRxiv · 10.1101/300228

Hyper-excitability and hyper-plasticity disrupt cerebellar signal transfer in the IB2 KO mouse model of autism

D'Angelo, E. U.·Soda, T.·Mapelli, L.·Locatelli, F.·Botta, L.·Goldfarb, M.·Prestori, F.
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Abstract

Autism spectrum disorders (ASD) are pervasive neurodevelopmental conditions that often involve mutations affecting synaptic mechanisms. Recently, the involvement of cerebellum in ASD has been suggested but the underlying functional alterations remained obscure. We investigated single-neuron and microcircuit properties in IB2 KO mice, which present a cerebellar phenotype associated with ASD. Granule cells showed a larger NMDA receptor-mediated current and enhanced intrinsic excitability raising the excitatory/inhibitory balance. Furthermore, the spatial organization of granular layer responses to mossy fibers shifted from a Mexican hat to stovepipe hat profile, with stronger excitation in the core and weaker inhibition in the surround. Finally, the size and extension of long-term synaptic plasticity was remarkably increased. These results show for the first time that hyper-excitability and hyper-plasticity disrupt signal transfer in the granular layer of IB2 KO mice supporting cerebellar involvement in the pathogenesis of ASD.

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D'Angelo, E. U., Soda, T., Mapelli, L., Locatelli, F., Botta, L., Goldfarb, M., Prestori, F.. 2018-04-12. Hyper-excitability and hyper-plasticity disrupt cerebellar signal transfer in the IB2 KO mouse model of autism. https://doi.org/10.1101/300228

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