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bioRxiv · 10.1101/298646

How to overcome the ATRA resistance with the 9aaTAD activation domains in retinoic acid receptors

Piskacek, M.·Havelka, M.·Knight, A.
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Abstract

In higher metazoa, the nuclear hormone receptors activate transcription trough their specific adaptors, nuclear hormone receptor cofactors NCoA, which are surprisingly absent in lower metazoa. In this study, we demonstrated that the 9aaTAD from NHR-49 receptor activates transcription as a small peptide. We showed, that the 9aaTAD domains are conserved in the human nuclear hormone receptors including HNF4, RARa, VDR and PPARg. The small 9aaTAD peptides derived from these nuclear hormone receptors also effectively activated transcription and that in absence of the NCoA adaptors. We identified adjacent inhibitory domains in the human HNF4 and RARa, which hindered their activation function.\n\nIn acute promyelocytic leukaemia (PML-RARa), the receptor mutations often caused all-trans retinoic acid (ATRA) resistance. The fact that almost the entire receptor is needed for ATRA mediated receptor activation, this activation pathway is highly susceptible for loss of function when mutated. Nevertheless in the most of the reported mutants, the activation domains 9aaTAD are still intact. The release of activation 9aaTAD from its dormancy by a new drug might be the sound strategy in combat the ATRA resistance in PML leukaemia.\n\nGraphical Abstract\n\nO_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=83 SRC=\"FIGDIR/small/298646_ufig1.gif\" ALT=\"Figure 1\">\nView larger version (36K):\norg.highwire.dtl.DTLVardef@187575borg.highwire.dtl.DTLVardef@782db0org.highwire.dtl.DTLVardef@1ff9fe7org.highwire.dtl.DTLVardef@15b27ee_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Piskacek, M., Havelka, M., Knight, A.. 2018-04-10. How to overcome the ATRA resistance with the 9aaTAD activation domains in retinoic acid receptors. https://doi.org/10.1101/298646

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