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bioRxiv · 10.1101/289686

Oblique plane single-molecule localization microscopy for thick samples

Kim, J.·Wojcik, M.·Wang, Y.·Xu, K.·Zhang, X.
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Abstract

We introduce single-molecule oblique plane microscopy (obSTORM) to directly image oblique sections of thick samples into depth without lengthy axial stack acquisition. Using oblique light-sheet illumination and oblique fluorescence detection, obSTORM offers uniform super-resolution throughout imaging depth in diverse biological specimens from cells to tissues. In particular, we demonstrate an isotropic resolution of [~]51 nm over a depth of 32 m for a tissue sample, and comparable resolution over a depth of 100 m using fluorescent beads.

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Kim, J., Wojcik, M., Wang, Y., Xu, K., Zhang, X.. 2018-03-27. Oblique plane single-molecule localization microscopy for thick samples. https://doi.org/10.1101/289686

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