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bioRxiv · 10.1101/285429

Y chromosomal noncoding RNA regulates autosomal gene expression via piRNAs in mouse testis

Reddy, H. M.·Bhattacharya, R.·Jehan, Z.·Mishra, K.·Annapurna, P.·Tiwari, S.·Praveena, N. M.·Alex, J. L.·Dhople, V. M.·Singh, L.·Sivaramakrishnan, M.·Chaturvedi, A.·Rangaraj, N.·Thomas, S. M.·Sridevi, B.·Kumar, S.·Dereddi, R. R.·Rayabandla, S.·Jesudasan, R. A.
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Abstract

Majority of the genes expressed during spermatogenesis are autosomal. Mice with different deletions of Yq show sub-fertility, sterility and sperm abnormalities. The connection between Yq deletion and autosomal gene regulation is not well understood. We describe a novel mouse Yq-derived long noncoding RNA, Pirmy, which shows unprecedented number of splice variants in testis. Further, Pirmy transcript variants act as templates for several piRNAs. We identified ten differentially expressed autosome-encoded sperm proteins in mutant mice. Pirmy transcript variants have homology to 5/3UTRs of these deregulated autosomal genes. Thus, subfertility in Y-deleted mice appears to be a polygenic phenomenon that is partially regulated epistatically by the Y-chromosome. Our study provides novel insights into possible role of MSY-derived ncRNAs in male fertility and reproduction. Finally, sperm phenotypes from the Y-deleted mice seem to be similar to that reported in inter-specific male-sterile hybrids. Taken together, this study provides novel insights into possible role of Y-derived ncRNAs in male sterility and speciation.

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Reddy, H. M., Bhattacharya, R., Jehan, Z., Mishra, K., Annapurna, P., Tiwari, S., Praveena, N. M., Alex, J. L., Dhople, V. M., Singh, L., Sivaramakrishnan, M., Chaturvedi, A., Rangaraj, N., Thomas, S. M., Sridevi, B., Kumar, S., Dereddi, R. R., Rayabandla, S., Jesudasan, R. A.. 2018-03-24. Y chromosomal noncoding RNA regulates autosomal gene expression via piRNAs in mouse testis. https://doi.org/10.1101/285429

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