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bioRxiv · 10.1101/281915

Meta-microRNAs as potential noninvasive markers for early diagnosis of Alzheimer’s disease

Jiang, N.·Ruan, C.-J.·Cheng, X.-R.·Wang, L.-N.·Tan, J.-P.·Wang, W.-S.·Liu, F.·Zhou, W.-X.·Zhang, Y.-X.
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Abstract

IntroductionThe aim of this study was to investigate the potential role of a microRNA panel as early diagnostic markers for Alzheimers disease (AD).\n\nMethodsThe differentially expressed serum microRNAs were screened with microarray among cognitively normal controls (CNC), mild cognitive impairment (MCI), and AD. QRT-PCR assay was applied to evaluate differentially expressed microRNAs with two independent cohorts including 202 participants. Logistic regression model based on microRNA panel was constructed using a training cohort and then validated using an independent cohort.\n\nResultsFirst, four differentially expressed serum microRNAs (let-7g, miR-197, miR-126 and miR-29a) were found, which expressions were positively correlated with mini mental state examination (MMSE) score. Second, a microRNA panel with the four microRNAs demonstrated good diagnostic performance for MCI and AD with 84% and 92% accuracy. Third, when combined with MMSE score, the diagnostic performance of the microRNA panel was further improved.\n\nDiscussionBlood microRNAs are potential AD biomarkers that may lead to new diagnostic strategies.

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Jiang, N., Ruan, C.-J., Cheng, X.-R., Wang, L.-N., Tan, J.-P., Wang, W.-S., Liu, F., Zhou, W.-X., Zhang, Y.-X.. 2018-03-14. Meta-microRNAs as potential noninvasive markers for early diagnosis of Alzheimer’s disease. https://doi.org/10.1101/281915

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