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bioRxiv · 10.1101/275321

Direct Evidence of Effect of Glycerol on Hydration and Helix-to-Sheet Transition of Myoglobin

Hirai, M.·Ajito, S.·Sugiyama, M.·Iwase, H.·Takata, S.-i.·Shimizu, N.·Igarashi, N.·Martel, A.·Porcar, L.
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Abstract

By using wide-angle X-ray scattering (WAXS), small-angle neutron scattering, and theoretical scattering function simulation, we have clarified the effect of glycerol on both the thermal structure transition and the hydration-shell of myoglobin. At the glycerol concentration, [≤] [~]40 % v/v, the decreasing tendency in the maximum dimension and the radius of gyration was observed by X-ray scattering. The neutron scattering result using the inverse contrast variation method directly shows the preservation of the hydration-shell density at the concentration [≤] [~]40 % v/v. This phenomenon is reasonably explained by the preferential exclusion of glycerol from the protein surface to preserve the hydration shell, as suggested by the previous studies. While, at the concentration, [≥] 50 % v/v, the opposite tendency was observed. It suggests the preferential solvation (partial preferential penetration or replacement of glycerol into or with hydration-shell water surrounding the protein surface) occurs at the higher concentration. The observed WAXS scattering data covers the distinct hierarchical structural levels of myoglobin structure ranging from the tertiary structure to the secondary one. Therefore, we have clarified the effect of glycerol on the thermal structural stability myoglobin at different hierarchical structural levels separately. Against the temperature rise, the structural transition temperatures for all hierarchical structural levels were elevated. Especially, the tertiary structure of myoglobin was more stabilized compared with the internal-structure and the helix-to-cross transition. It suggests that the protective action of glycerol on protein structures essentially results from the preservation of the preferential hydration-shell of proteins.

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Hirai, M., Ajito, S., Sugiyama, M., Iwase, H., Takata, S.-i., Shimizu, N., Igarashi, N., Martel, A., Porcar, L.. 2018-03-08. Direct Evidence of Effect of Glycerol on Hydration and Helix-to-Sheet Transition of Myoglobin. https://doi.org/10.1101/275321

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