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bioRxiv · 10.1101/273359

High Numerical Aperture Epi-illumination Selective Plane Illumination Microscopy

Yang, B.·Wang, Y.·Feng, S.·Pessino, V.·Stuurman, N.·Huang, B.
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Abstract

Selective-plane illumination microscopy (SPIM) provides unparalleled advantages for long-term volumetric imaging of living organisms. In order to achieve high-resolution imaging in common biological sample holders, we designed a high numerical aperture (NA) epi-illumination SPIM (eSPIM) system, which utilizes a single objective and has an identical sample interface as an inverted fluorescence microscope with no additional reflection elements. This system has an effective detection NA of > 1.06. We demonstrated multicolor and fast volumetric imaging of live cells and single-molecule super-resolution microscopy using our system.

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Yang, B., Wang, Y., Feng, S., Pessino, V., Stuurman, N., Huang, B.. 2018-02-28. High Numerical Aperture Epi-illumination Selective Plane Illumination Microscopy. https://doi.org/10.1101/273359

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