Verified the effectiveness of AsCpf1 system in a variety of vertebrate species

Li, Z.; Shi, Z.; Fan, N.; Chen, Y.; Guo, J.; Wu, J.; Song, H.; Chu, S.; Mo, K.; Zhao, B.; Ouyang, Z.; Tian, D.; Zhao, S.; Zhu, J.; Chen, J.; Lai, L.; Pei, D.

doi:10.1101/272716

bioRxiv · 10.1101/272716

Verified the effectiveness of AsCpf1 system in a variety of vertebrate species

Li, Z.·Shi, Z.·Fan, N.·Chen, Y.·Guo, J.·Wu, J.·Song, H.·Chu, S.·Mo, K.·Zhao, B.·Ouyang, Z.·Tian, D.·Zhao, S.·Zhu, J.·Chen, J.·Lai, L.·Pei, D.

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Abstract

CRISPR/Cpf1 system is a novel genomic editing tool. Because of its more sophisticated components, and lower off-target rate, it has the potential to become a better gene-editing tool. Previous reports showed that CRISPR/Cpf1 could work effectively in multiple species. But our data show that AsCpf1 activity has a big difference in different vertebrates. Using in vitro experiments, we finally learned that the difference between species is due to temperature.

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Li, Z., Shi, Z., Fan, N., Chen, Y., Guo, J., Wu, J., Song, H., Chu, S., Mo, K., Zhao, B., Ouyang, Z., Tian, D., Zhao, S., Zhu, J., Chen, J., Lai, L., Pei, D.. 2018-02-27. Verified the effectiveness of AsCpf1 system in a variety of vertebrate species. https://doi.org/10.1101/272716

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