A fully automatic method yielding initial models from high-resolution electron cryo-microscopy maps

Terwilliger, T. C.; Adams, P. D.; Afonine, P. V.; Sobolev, O. V.

doi:10.1101/267138

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bioRxiv · 10.1101/267138

A fully automatic method yielding initial models from high-resolution electron cryo-microscopy maps

Terwilliger, T. C.·Adams, P. D.·Afonine, P. V.·Sobolev, O. V.

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Abstract

A fully automated procedure for optimization and interpretation of reconstructions from cryo-EM is developed and applied to 476 datasets with resolution of 4.5 [A] or better, including reconstructions of 47 ribosomes and 32 other protein-RNA complexes. The median fraction of residues in the deposited structures reproduced automatically was 71% for reconstructions determined at resolutions of 3 [A] or better and 47% for those at lower resolution.

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Terwilliger, T. C., Adams, P. D., Afonine, P. V., Sobolev, O. V.. 2018-02-16. A fully automatic method yielding initial models from high-resolution electron cryo-microscopy maps. https://doi.org/10.1101/267138

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