Detection and Degradation of Nonsense-mediated mRNA Decay Substrates Involve Two Distinct Upf1-bound Complexes

Dehecq, M.; Decourty, L.; Namane, A.; Proux, C.; Kanaan, J.; Le Hir, H.; Jacquier, A.; Saveanu, C.

doi:10.1101/266833

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bioRxiv · 10.1101/266833

Detection and Degradation of Nonsense-mediated mRNA Decay Substrates Involve Two Distinct Upf1-bound Complexes

Dehecq, M.·Decourty, L.·Namane, A.·Proux, C.·Kanaan, J.·Le Hir, H.·Jacquier, A.·Saveanu, C.

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Abstract

Nonsense-mediated mRNA decay (NMD) is a translation-dependent RNA degradation pathway involved in many cellular pathways and crucial for telomere maintenance and embryo development. Core NMD factors Upf1, Upf2 and Upf3 are conserved from yeast to mammals, but a universal NMD model is lacking. We used affinity purification coupled with mass spectrometry and an improved data analysis protocol to obtain the first large-scale quantitative characterization of yeast NMD complexes in yeast (112 experiments). Unexpectedly, we identified two distinct complexes associated with Upf1: Detector (Upf1/2/3) and Effector. Effector contained the mRNA decapping enzyme, together with Nmd4 and Ebs1, two proteins that globally affected NMD and were critical for RNA degradation mediated by the Upf1 C-terminal helicase region. The fact that Nmd4 association to RNA was dependent on Detector components and the similarity between Nmd4/Ebs1 and mammalian Smg5-7 proteins suggest that in all eukaryotes NMD operates through successive Upf1-bound Detector and Effector complexes.

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Dehecq, M., Decourty, L., Namane, A., Proux, C., Kanaan, J., Le Hir, H., Jacquier, A., Saveanu, C.. 2018-02-16. Detection and Degradation of Nonsense-mediated mRNA Decay Substrates Involve Two Distinct Upf1-bound Complexes. https://doi.org/10.1101/266833

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