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bioRxiv · 10.1101/264978

Axenic Aedes aegypti develop without live bacteria, but exhibit delayed development and reduced oviposition.

Correa, M. A.·Brackney, D. E.·Steven, B.
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Abstract

The mosquito gut microbiome plays an important role in mosquito development and fitness, providing a promising avenue for novel mosquito control strategies. Here we present a method for rearing axenic (bacteria free) Aedes aegypti mosquitoes, which will greatly facilitate mechanistic studies documenting the structure and function of the microbiome. Through feeding sterilized larvae agar plugs containing attenuated Escherichia coli, mosquito development was observed in the absence of living bacteria. Axenic larvae were capable of full development into adults, which laid eggs that were subsequently hatched. However, axenic mosquitoes exhibited delayed development time and reduced egg clutch size in comparison to bacterially colonized mosquitoes. These findings suggest that mosquito development is not dependent on live bacteria, but their phenotype is modulated by the presence of microorganisms. This axenic system offers a new tool in which the mosquito microbiome can be systematically manipulated for a deeper understanding of microbiome host interactions.

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Correa, M. A., Brackney, D. E., Steven, B.. 2018-02-13. Axenic Aedes aegypti develop without live bacteria, but exhibit delayed development and reduced oviposition.. https://doi.org/10.1101/264978

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