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bioRxiv · 10.1101/264788

An integrated approach to comprehensively map the molecular context of proteins

Liu, X.·Salokas, K.·Tamene, F.·Varjosalo, M.
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Abstract

Protein-protein interactions underlie almost all cellular functions. The comprehensive mapping of these complex cellular networks of stable and transient associations has been made available by affinity purification mass spectrometry (AP-MS) and more recently by proximity based labelling methods such as BioID. Due the advancements in both methods and MS instrumentation, an in-depth analysis of the whole human proteome is at grasps. In order to facilitate this, we designed and optimized an integrated approach utilizing MAC-tag combining both AP-MS and BioID in a single construct. We systematically applied this approach to 18 subcellular localization markers and generated a molecular context database, which can be used to define molecular locations for any protein of interest. In addition, we show that by combining the AP-MS and BioID results we can also obtain interaction distances within a complex. Taken together, our combined strategy offers comprehensive approach for mapping physical and functional protein interactions.

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Liu, X., Salokas, K., Tamene, F., Varjosalo, M.. 2018-02-13. An integrated approach to comprehensively map the molecular context of proteins. https://doi.org/10.1101/264788

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