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bioRxiv · 10.1101/261594

High-quality ultrastructural preservation using cryofixation for 3D electron microscopy of genetically labeled tissues

Tsang, T. K.·Bushong, E. A.·Boassa, D.·Hu, J.·Romoli, B.·Phan, S.·Dulcis, D.·Su, C.-Y.·Ellisman, M. H.
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Abstract

Electron microscopy (EM) offers unparalleled power to study cell substructures at the nanoscale. Cryofixation by high-pressure freezing offers optimal morphological preservation, as it captures cellular structures instantaneously in their near-native states. However, the applicability of cryofixation is limited by its incompatibilities with diaminobenzidine labeling using genetic EM tags and the high-contrast en bloc staining required for serial block-face scanning electron microscopy (SBEM). In addition, it is challenging to perform correlated light and electron microscopy (CLEM) with cryofixed samples. Consequently, these powerful methods cannot be applied to address questions requiring optimal morphological preservation and high temporal resolution. Here we developed an approach that overcomes these limitations; it enables genetically labeled, cryofixed samples to be characterized with SBEM and 3D CLEM. Our approach is broadly applicable, as demonstrated in cultured cells, Drosophila olfactory organ and mouse brain. This optimization exploits the potential of cryofixation, allowing quality ultrastructural preservation for diverse EM applications.

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Tsang, T. K., Bushong, E. A., Boassa, D., Hu, J., Romoli, B., Phan, S., Dulcis, D., Su, C.-Y., Ellisman, M. H.. 2018-02-07. High-quality ultrastructural preservation using cryofixation for 3D electron microscopy of genetically labeled tissues. https://doi.org/10.1101/261594

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