A multi-omic atlas of the human frontal cortex for aging and Alzheimer's disease research

De Jager, P.; Ma, Y.; McCabe, C.; Xu, J.; Vardarajan, B. N.; Felsky, D.; Klein, H.-U.; White, C. C.; Peters, M. A.; Lodgson, B.; Nejad, P.; Tang, A.; Mangravite, L. M.; Yu, L.; Gaiteri, C.; Mostafavi, S.; Schneider, J. A.; Bennett, D. A.

doi:10.1101/251967

bioRxiv · 10.1101/251967

A multi-omic atlas of the human frontal cortex for aging and Alzheimer's disease research

De Jager, P.·Ma, Y.·McCabe, C.·Xu, J.·Vardarajan, B. N.·Felsky, D.·Klein, H.-U.·White, C. C.·Peters, M. A.·Lodgson, B.·Nejad, P.·Tang, A.·Mangravite, L. M.·Yu, L.·Gaiteri, C.·Mostafavi, S.·Schneider, J. A.·Bennett, D. A.

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Abstract

We initiated the systematic profiling of the dorsolateral prefrontal cortex obtained from a subset of autopsied individuals enrolled in the Religious Orders Study (ROS) or the Rush Memory and Aging Project (MAP), which are jointly designed and belong to a very few prospective studies of aging and dementia with detailed, longitudinal cognitive phenotyping during life and a quantitative, structured neuropathologic examination after death for >3,322 subjects. Here, we outline the first generation of data including genome-wide genotypes (n=2,090), whole genome sequencing (n=1,179), DNA methylation (n=740), chromatin immunoprecipitation with sequencing using an anti-Histone 3 Lysine 9 acetylation (H3K9Ac) antibody (n=712), RNA sequencing (n=638), and miRNA profile (n=702). Generation of other omic data including ATACseq, proteomic and metabolomics profiles is ongoing. Thanks to its prospective design and recruitment of older, non-demented individuals, these data can be repurposed to investigate a large number of syndromic and quantitative neuroscience phenotypes. The many subjects that are cognitively non-impaired at death also offer insights into the biology of the human brain in older non-impaired individuals.

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De Jager, P., Ma, Y., McCabe, C., Xu, J., Vardarajan, B. N., Felsky, D., Klein, H.-U., White, C. C., Peters, M. A., Lodgson, B., Nejad, P., Tang, A., Mangravite, L. M., Yu, L., Gaiteri, C., Mostafavi, S., Schneider, J. A., Bennett, D. A.. 2018-01-23. A multi-omic atlas of the human frontal cortex for aging and Alzheimer's disease research. https://doi.org/10.1101/251967

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