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bioRxiv · 10.1101/251561

In vivo multiphoton microscopy of cardiomyocyte calcium dynamics in the beating mouse heart

Small, D. M.·Jones, J. S.·Nishimura, N.
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Abstract

We demonstrated intravital multiphoton microscopy in the beating heart in an intact mouse and optically measured action potentials with GCaMP6f, a genetically-encoded calcium indicator. Images were acquired at 30 fps with spontaneous heart beat and continuously running ventilated breathing. The data were reconstructed into three-dimensional volumes showing tissue structure, displacement, and GCaMP activity in cardiomyocytes as a function of both the cardiac and respiratory cycle.

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Small, D. M., Jones, J. S., Nishimura, N.. 2018-01-22. In vivo multiphoton microscopy of cardiomyocyte calcium dynamics in the beating mouse heart. https://doi.org/10.1101/251561

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