Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution

Wang, H.; Zhu, Q.; Ding, L.; Shen, Y.; Yang, C.-Y.; Xu, F.; Shu, C.; Guo, Y.; Xiong, Z.; Shan, Q.; Jia, F.; Su, P.; Yang, Q.-R.; Li, B.; He, X.; Chen, X.; Wu, F.; Zhou, J.-N.; Han, H.; Lau, P.-M.; Bi, G.-Q.

doi:10.1101/240770

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bioRxiv · 10.1101/240770

Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution

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Abstract

We describe a new light-sheet microscopy method for fast, large-scale volumetric imaging. Combining synchronized scanning illumination and oblique imaging over cleared, thick tissue sections in smooth motion, our approach achieves high-speed 3D image acquisition of an entire mouse brain within 2 hours, at a resolution capable of resolving synaptic spines. It is compatible with immunofluorescence labeling, enabling flexible cell-type specific brain mapping, and is readily scalable for large biological samples such as primate brain.

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Wang, H., Zhu, Q., Ding, L., Shen, Y., Yang, C.-Y., Xu, F., Shu, C., Guo, Y., Xiong, Z., Shan, Q., Jia, F., Su, P., Yang, Q.-R., Li, B., He, X., Chen, X., Wu, F., Zhou, J.-N., Han, H., Lau, P.-M., Bi, G.-Q.. 2017-12-29. Scalable volumetric imaging for ultrahigh-speed brain mapping at synaptic resolution. https://doi.org/10.1101/240770

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