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bioRxiv · 10.1101/228296

Nestin in immature embryonic neurons regulates axon growth cone morphology and Sema3a sensitivity

Bott, C. J.·Johnson, C. G.·Yap, C. C.·Dwyer, N. D.·Newell-Litwa, K.·Winckler, B.
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Abstract

Correct wiring in the neocortex requires that responses to an individual guidance cue vary among neurons in the same location, and within the same neuron over time. Nestin is an atypical intermediate filament expressed highly in neural progenitors and is thus used widely as a progenitor marker. Here we show a subpopulation of embryonic cortical neurons which transiently express nestin in their axons. Nestin expression is thus not restricted to neural progenitors but persists at lower levels in some newborn neurons for 2-3 days. We found that nestin-expressing neurons have smaller growth cones, suggesting that nestin affects cytoskeletal dynamics. Nestin, unlike other intermediate filament subtypes, regulates cdk5 kinase. Cdk5 activity is induced by the repulsive guidance cue Sema3a leading to growth cone collapse in vitro. Therefore, we tested whether nestin-expressing neurons showed altered responses to Sema3a. We find that nestin-expressing newborn neurons are more sensitive to Sema3a in a roscovitine-sensitive manner, whereas nestin knockdown results in lowered sensitivity to Sema3a. We propose that nestin functions in immature neurons to modulate cdk5 and thereby the Sema3a response. Thus, the transient expression of nestin could allow for temporal modulation of a neuron's response to Sema3a particularly during early axon guidance decisions.

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Bott, C. J., Johnson, C. G., Yap, C. C., Dwyer, N. D., Newell-Litwa, K., Winckler, B.. 2017-12-03. Nestin in immature embryonic neurons regulates axon growth cone morphology and Sema3a sensitivity. https://doi.org/10.1101/228296

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