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bioRxiv · 10.1101/217166

SgTiler: a fast method to design tiling sgRNAs for CRISPR/Cas9 mediated screening

Ahmed, M.·He, H. H.
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Abstract

SummaryScreening of genomic regions of interest using CRISPR/Cas9 is getting increasingly popular. The system requires designing of single guide RNAs (sgRNAs) that can efficiently guide the Cas9 endonuclease to the targeted region with minimal off-target effects. Tiling sgRNAs is the most effective way to perturb regulatory regions, such as promoters and enhancers. sgTiler is the first tool that provides a fast method for designing tiling sgRNAs.\n\nAvailability and ImplementationsgTiler is a command line tool that requires only one command to execute. Its source code is freely available on the web at https://github.com/HansenHeLab/sgTiler. sgTiler is implemented in Python and supported on any platform with Python and Bowtie.

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Ahmed, M., He, H. H.. 2017-11-09. SgTiler: a fast method to design tiling sgRNAs for CRISPR/Cas9 mediated screening. https://doi.org/10.1101/217166

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