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bioRxiv · 10.1101/2025.11.24.690344

Modelling mental disorders in zebrafish. Neurexins severely modulate anxiety, social behaviours and aggression.

Nguyen, Q.·Guo, F.·Mowry, B.·Das, J.·Giacomotto, J.
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Abstract

Can we reproduce mental disorders in zebrafish? To explore this question, we manipulated the neurexin gene family. Neurexin (nrxn) genes encode synaptic cell-adhesion molecules that have been repetitively associated with neurodevelopmental and mental disorders. While the zebrafish animal model offers tremendous advantages for dissecting neural development/function, no robust nrxn zebrafish models are currently available. In this study, we generated the first collection of zebrafish knockout lines for each nrxn gene, with mutations ranging from transmembrane domain- to full genomic locus-deletions. Surprisingly, all homozygous lines developed normally, presenting no gross neurodevelopmental or obvious early behavioural abnormalities. However, this absence of early phenotypes translated into profound, paralog-specific behavioural alterations emerging during juvenile-to-adult stages. All neurexin knockouts affected mating behaviour, complicating the generation and maintenance of homozygous lines. Except for this shared behavioural alteration, nrxn1, but not nrxn2 or nrxn3, led to marked changes in social behaviour and aggression. In contrast, nrxn2 mutants exhibited severe anxiety-like behaviours, including bottom-dwelling and repetitive freezing/seizure events. Strikingly, nrxn1 full-locus deletion mutants showed opposing behaviour, spending most of their time near the surface. The two also displayed opposite responses to open/closed field transitions; confinement alleviated nrxn2 anxiety but enhanced nrxn1 surface-dwelling. Meanwhile, nrxn3 mutants behaved normally in all our initial tests. In summary, our study introduces a complete set of zebrafish mutants covering the whole nrxn gene family, presenting striking juvenile/adult behavioural alterations despite the absence of noticeable early defects; echoing the delayed onset of human psychiatric disorders such as schizophrenia. This work demonstrates the value of zebrafish to study mental disorders and unlock a novel platform to unravel the pathogenic contribution of neurexin and associated subtle neurodevelopmental changes/timing that drive the emergence of mental illnesses.

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Nguyen, Q., Guo, F., Mowry, B., Das, J., Giacomotto, J.. 2025-11-25. Modelling mental disorders in zebrafish. Neurexins severely modulate anxiety, social behaviours and aggression.. https://doi.org/10.1101/2025.11.24.690344

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