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bioRxiv · 10.1101/2025.11.13.688015

PROX1 loss in adult mouse Schlemm's canal causes permanent ocular hypertension

Abstract

Glaucoma is associated with ocular hypertension and lowering intraocular pressure is a key objective of glaucoma therapies. Recent studies have established a role for the Schlemms canal endothelium in this pressure increase and have shown it to have a unique, lymphatic-like, hybrid phenotype. However, the role of these lymphatic phenotypes in the adult canal remains uncertain. Long-term functional studies have been limited by systemic importance of lymphatic genes and lack of Schlemms canal-specific animal models. Here, we designed and validated a strategy using 4OH-tamoxifen-loaded nanocarriers to generate targeted, Schlemms canal specific knockout mice lacking lymphatic phenotypes. Using this system, we selectively deleted Prox1, the master transcription factor governing lymphatic fate. Within four weeks, intraocular pressure significantly increased, and ocular hypertension was maintained for at least 24 weeks. Unlike lymphatic vessels, which degenerate following Prox1 deletion, Schlemms canal reverted to a less functional vein-like phenotype with no change in size or morphology. These results highlight the utility of nanocarriers for tissue-specific genetic recombination and demonstrate that changes in lymphatic phenotypes alter intraocular pressure, providing new targets for glaucoma therapy. Moreover, as we found that PROX1 was downregulated with age in human Schlemms canal, these canal-specific conditional Prox1 knockout mice are a valuable new adult-onset model of ocular hypertension that captures key features of age-related human disease.

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BibTeXRIS

Ochoa, S. L., Li, H. L., Kim, H., Yan, Z., Mendonca, N. C., Liu, P., Lee, H. J., Vincent, M. P., Zhang, H. F., Gong, H., Scott, E. A., Johnson, M., Thomson, B. R.. 2025-11-14. PROX1 loss in adult mouse Schlemm's canal causes permanent ocular hypertension. https://doi.org/10.1101/2025.11.13.688015

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