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bioRxiv · 10.1101/2025.10.31.685793

Structural basis of HSV-1 DNA replication origin-binding protein UL9

Huang, C.·Wu, H.·Song, J.·Zhang, X.·Ma, J.
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Abstract

The herpesvirus DNA replication machinery comprises a battery of viral enzymes that orchestrate viral genome synthesis. In herpes simplex virus type 1 (HSV-1), the machinery consists of seven essential components, including the origin-binding protein UL9, the single-stranded DNA (ssDNA)-binding protein ICP8, the heterodimeric DNA polymerase complex UL30-UL42, and the heterotrimeric helicase-primase complex UL5-UL8-UL52. UL9, a superfamily 2 (SF2) helicase, functions as a dimer that specifically recognizes replication origins and unwinds duplex DNA to initiate replication. Furthermore, UL9 recruits the replication machinery through interactions with viral components and engages cellular proteins that regulate its function. However, the molecular mechanisms underlying UL9s multifunctionality remain incompletely understood due to the lack of structural information. Here, we present cryo-electron microscopy structures of UL9 in both apo and DNA-bound states. Together with biochemical and enzymatic assays, we elucidate the molecular basis of UL9 dimerization, origin recognition and allosteric regulation by ICP8.

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Huang, C., Wu, H., Song, J., Zhang, X., Ma, J.. 2025-10-31. Structural basis of HSV-1 DNA replication origin-binding protein UL9. https://doi.org/10.1101/2025.10.31.685793

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