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bioRxiv · 10.1101/2025.10.30.685603

Rationally designed minimized TbpB confers broad protection against meningococcal infection

Abstract

Transferrin binding protein B (TbpB), an iron acquisition protein, has long been recognized as a promising vaccine candidate targeting the pathogenic Neisseria species, including Neisseria meningitidis, the cause of meningococcal disease, and Neisseria gonorrhoeae, the cause of gonorrhea. A challenge to the development of this protein as a vaccine immunogen is the extent of antigenic variability it exhibits, which complicates the selection of a single variant to elicit a broadly cross-protective immune response. We have utilized structure-informed antigen engineering to develop a minimized version of TbpB consisting of the proteins carboxy-terminal lobe with its variable surface loops removed. Here, we reveal the effectiveness of this "loopless C-lobe" as an independent immunogen, with structural characterization and stability studies to demonstrate its integrity, and murine immunization and challenge studies that establish its ability to elicit robust protective efficacy by using N. meningitidis invasive infection and nasopharyngeal colonization models. The breadth of protection provided, as measured by both in vitro analysis and cross-protection mouse challenge studies, indicate that a single loopless C-lobe elicits a broadly cross-protective immune response against the diverse panel of meningococcal strains tested, and that the cross-reactivity is superior to that offered by the intact TbpB or the native C-lobe. Together, this study demonstrates the utility of structure-informed antigen engineering towards the development of broadly efficacious protein-based vaccines. ImportanceSurface-exposed proteins on bacterial pathogens are enticing candidate vaccine targets, however their exposure to the immune system frequently leads to high levels of antigenic variation, a factor that complicates the development of broadly protective vaccines. Here, we undertake an antigen engineering approach to develop a minimized version of a surface lipoprotein, transferrin binding protein B, where variable regions of the protein have been removed to focus the immune response to conserved regions of this antigen. We combine structural studies and mouse infection models of Neisseria meningitidis, the cause of meningococcal disease, and Neisseria gonorrhoeae, the causative agent of gonorrhea, to reveal that our strategic minimizing of the protein immunogen focuses the immune response to extend the resulting breadth of cross-reactivity and cross-protection.

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BibTeXRIS

Islam, E. A., Fegan, J. E., Cole, G. B., Calmettes, C., Ng, D., Au, N. Y. T., Buckwalter, C. M., Ahn, S. K., Curran, D. M., Caruso, L.-l., Schryvers, A. B., Moraes, T., Gray-Owen, S.. 2025-10-31. Rationally designed minimized TbpB confers broad protection against meningococcal infection. https://doi.org/10.1101/2025.10.30.685603

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