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bioRxiv · 10.1101/2025.10.25.684418

Evaluation of Paper-based Loop-Mediated Isothermal Amplification

Kim, T.·Palla, G.·Raut, B.·Verma, M. S.·Ardekani, A. M.
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Abstract

Colorimetric loop-mediated isothermal amplification (LAMP) on microfluidic paper-based analytical devices (PADs) offers a low-cost, disposable, and equipment-free alternative to liquid LAMP assays. However, amplification on PADs is consistently slower, by 5-46%, than reactions in tubes. To identify the origin of this delay, we evaluated heat transfer, diffusion in porous cellulose, and nonspecific adsorption of LAMP components across both high- and low-copy input regimes. Our results show that once thermal equilibrium is reached, reduced effective diffusion is the dominant contributor to the kinetic lag at low copy numbers, whereas nonspecific adsorption becomes the primary barrier at higher template concentrations. Pre-coating the paper with bovine serum albumin (BSA) mitigates adsorption. It narrows the tube-to-paper gap, thereby accelerating amplification of the SARS-CoV-2 ORF7ab synthetic gene by an average of 6 minutes, from 1E3 to 1E5 copies per reaction. These findings provide a mechanistic basis for the copy-number-dependent behavior of PAD LAMP and offer simple, low-cost strategies to improve the speed and reliability of PAD nucleic acid assays.

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Kim, T., Palla, G., Raut, B., Verma, M. S., Ardekani, A. M.. 2025-10-26. Evaluation of Paper-based Loop-Mediated Isothermal Amplification. https://doi.org/10.1101/2025.10.25.684418

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