Temperature modulates PFAS accumulation and energy allocation in sheepshead minnows

Grimmelpont, M.; Rodgers, M. L.; Levin, M.; De Guise, S.; Agrawal, A.; Baron, J.; Bolnick, D. I.; Milligan-McClellan, K.; Provotas, A. A.; Brandt, J. E.

doi:10.1101/2025.10.23.684267

bioRxiv · 10.1101/2025.10.23.684267

Temperature modulates PFAS accumulation and energy allocation in sheepshead minnows

Grimmelpont, M.·Rodgers, M. L.·Levin, M.·De Guise, S.·Agrawal, A.·Baron, J.·Bolnick, D. I.·Milligan-McClellan, K.·Provotas, A. A.·Brandt, J. E.

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Abstract

Climate warming and chemical pollution shape aquatic ecosystems, yet the physiological mechanisms underlying their combined effects remain unclear. We investigated how projected increases in mean summer surface water temperature alter per- and polyfluoroalkyl substances (PFAS) toxicokinetics and their effects on sheepshead minnows (Cyprinodon variegatus) physiological performance. Adult fish were chronically exposed to an environmentally relevant PFAS mixture (perfluorooctane sulfonate (PFOS) + perfluorooctanoate (PFOA)) under current and projected mean-temperature scenarios. Tissue PFAS concentrations, whole-organism metabolic rates, swimming performance, reproductive parameters, somatic indices were assessed. Temperature modified PFAS tissue concentrations in a compound- and tissue-specific manner, promoting PFOA redistribution to eggs. Metabolic responses were temperature-dependent: at 26 {degrees}C, higher tissue PFAS concentrations were associated with elevated standard and maximum metabolic rates (SMR and MMR), maintaining aerobic scope (AS). At 28.5 {degrees}C, SMR remained stable while MMR and AS declined with rising PFAS, indicating less oxygen for energetically demanding activities. Despite unchanged swimming and reproductive outputs, an increased hepatosomatic index with increasing tissue PFAS concentrations and altered PFAS distribution suggest detoxification costs. These findings indicate that increases in mean water temperature are likely to exacerbate contaminant stress, with consequences for coastal fish population resilience and offspring development. PFAS risk assessment should consider co-stressors under projected warming. SynopsisLimited research addresses how temperature affects PFAS toxicokinetics and toxicity. This study shows that warming reshapes tissue PFAS concentrations and distribution, and influences fish energy-allocation trade-offs.

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Grimmelpont, M., Rodgers, M. L., Levin, M., De Guise, S., Agrawal, A., Baron, J., Bolnick, D. I., Milligan-McClellan, K., Provotas, A. A., Brandt, J. E.. 2025-10-24. Temperature modulates PFAS accumulation and energy allocation in sheepshead minnows. https://doi.org/10.1101/2025.10.23.684267

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