Similar Fecal SCFA Patterns Despite Diverse Gut Microbiota in Polish and Japanese Children During the First Two Years of Life. The longitudinal, comparative, validated study.

Łoniewski, I.; Kaczmarczyk, M.; Podsiadło, K.; Cembrowska-Lech, D.; Löber, U.; Bücking, R.; Skonieczna-Zydecka, K.; Forslund-Startceva, S. K.; Łoniewska, B.

doi:10.1101/2025.10.23.684084

bioRxiv · 10.1101/2025.10.23.684084

Similar Fecal SCFA Patterns Despite Diverse Gut Microbiota in Polish and Japanese Children During the First Two Years of Life. The longitudinal, comparative, validated study.

Łoniewski, I.·Kaczmarczyk, M.·Podsiadło, K.·Cembrowska-Lech, D.·Löber, U.·Bücking, R.·Skonieczna-Zydecka, K.·Forslund-Startceva, S. K.·Łoniewska, B.

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Abstract

BackgroundEarly-life gut microbiome assembly is shaped by environment, diet, and perinatal exposures. Whether population context shifts taxonomic trajectories and functional outputs similarly remains unclear. MethodsWe re-analyzed longitudinal infant cohorts from Poland (PL) and Japan (JP) across five time points (1 week, 1 month, 6 months, 1 year, 2 years). Amplicon sequence variants (ASVs) were processed uniformly. Cross-sectional alpha diversity (richness, evenness, Shannon, Simpson) was modeled with covariate adjustment (sex, antibiotics, diet). Beta diversity used Bray-Curtis distances (PCoA) and PERMANOVA (unadjusted/adjusted). Confounder-aware genus-level differential abundance identified features not reducible to covariates. Longitudinal genus trajectories and genus-SCFA (acetate, propionate, butyrate, isobutyrate) associations were tested using mixed-effects models. SCFAs were z-score-normalized to harmonize units across cohorts. Sensitivity analyses restricted PL to vaginally delivered infants. Finnish (FIN) and United States (US) cohorts were included for taxonomic validation. ResultsEvenness, Shannon, and Simpson were similar between PL and JP at most time points; richness was higher in PL at 1 week with trends at 6 months. Beta diversity showed a significant country effect at every time point except 1 month, robust to covariate adjustment and to restriction to 74 genera shared between PL and JP. Genus-level differential abundance yielded 33 features (26 enriched in PL, 7 in JP) without consistent cross-time recurrence. Longitudinally, most genus trajectories were cohort-specific; genus-SCFA associations were largely population-specific (few overlaps for butyrate/isobutyrate, none for acetate/propionate). Despite taxonomic and association differences, SCFA trajectories did not differ between cohorts after harmonization and adjustment. FIN/US validation supported the robustness of temporal taxonomic signals with few discordances. ConclusionsTaxonomic profiles and genus-SCFA relationships diverge across populations, whereas core metabolic outputs (fecal SCFAs) follow a conserved, resilient trajectory in early life. This "divergent taxa, convergent function" pattern suggests early-life interventions should prioritize functional maturation over targeting specific taxa. Broader multi-omic studies integrating growth, immune, and neurodevelopmental outcomes are warranted.

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Łoniewski, I., Kaczmarczyk, M., Podsiadło, K., Cembrowska-Lech, D., Löber, U., Bücking, R., Skonieczna-Zydecka, K., Forslund-Startceva, S. K., Łoniewska, B.. 2025-10-23. Similar Fecal SCFA Patterns Despite Diverse Gut Microbiota in Polish and Japanese Children During the First Two Years of Life. The longitudinal, comparative, validated study.. https://doi.org/10.1101/2025.10.23.684084

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