How much can reticulate evolution entangle plant systematics? Revisiting subfamilial classification of the Malvatheca clade (Malvaceae) on the basis of phylogenomics

Luna, G.; Costa, L.; Pezzini, F. F.; Karimi, N.; Strijk, J. S.; Carvalho-Sobrinho, J. G.; Colli-Silva, M.; Marques, A.; Souza, G.

doi:10.1101/2025.10.17.683170

bioRxiv · 10.1101/2025.10.17.683170

How much can reticulate evolution entangle plant systematics? Revisiting subfamilial classification of the Malvatheca clade (Malvaceae) on the basis of phylogenomics

Luna, G.·Costa, L.·Pezzini, F. F.·Karimi, N.·Strijk, J. S.·Carvalho-Sobrinho, J. G.·Colli-Silva, M.·Marques, A.·Souza, G.

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Abstract

Reticulate evolution (RE), involving hybridization and related processes, generates network-like rather than strictly bifurcating relationships among lineages and can obscure phylogenetic relationships. Detecting ancient hybridization is particularly challenging, as genomic signals may erode over time. The Malvatheca clade (Malvaceae), marked by multiple paleopolyploidy events since its estimated origin 66 my, offers a useful model for examining RE. Its three subfamilies--Bombacoideae (with high chromosome numbers, mostly trees), Malvoideae (lower chromosome numbers, mostly herbs), and the recently described Matisioideae--show unresolved relationships, with several taxa of uncertain placement. We conducted a phylogenomic analysis of 62 Malvatheca species via complete plastomes, 35S rDNA cistrons, and comparative repeatome data. All datasets consistently resolved three main clades: (i) Bombacoideae, (ii) Malvoideae, and (iii) a heterogeneous assemblage including most of the recently assigned Matisioideae and several incertae sedis taxa, suggesting a more complex evolutionary history and circumscription than previously recognized. Chromosome numbers were negatively correlated with repeatome diversity: Bombacoideae presented higher counts but lower repeat diversity, possibly reflecting slower repeat evolution associated with woody growth forms. In contrast, clade III showed marked heterogeneity in both chromosome number and repeat composition, which is consistent with a reticulate origin. Overall, our results show evidence of ancient hybridization and polyploidy, predominantly allopolyploidy, in shaping Malvatheca evolution. These results highlight that reticulation and genome dynamics, rather than taxonomic boundaries alone, are central to understanding the diversification of Malvatheca.

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Luna, G., Costa, L., Pezzini, F. F., Karimi, N., Strijk, J. S., Carvalho-Sobrinho, J. G., Colli-Silva, M., Marques, A., Souza, G.. 2025-10-18. How much can reticulate evolution entangle plant systematics? Revisiting subfamilial classification of the Malvatheca clade (Malvaceae) on the basis of phylogenomics. https://doi.org/10.1101/2025.10.17.683170

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