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bioRxiv · 10.1101/2025.10.09.681433

Single Root hair growth under constant force: insights into wall mechanics

Abstract

Tip growth is a tightly regulated process that enables root hairs to explore their surroundings, enhancing plant development, particularly by improving nutrient uptake. While Lockharts viscoplastic framework is widely used to describe this process, it has received limited experimental validation. By integrating optical microscopy with a custom microplate-based rheometer, we created a novel protocol to simultaneously measure, for individual growing root hairs, both the reduction in growth rate and the instantaneous compression in response to a step in applied axial force. The observed growth rate reduction aligns remarkably with a 1D Lockhart viscoplastic model, experimentally validating this framework in tip-growing cells. Additionally, the instantaneous compression upon force application provided an in situ estimate of turgor pressure. Together, these measurements allowed us to determine, for the first time in Arabidopsis root hairs, two critical parameters: the yield turgor pressure and cell wall viscosity. Our approach--including the technique, protocol, and analytical framework--can be readily adapted to other tip-growing species and diverse experimental conditions (e.g., varying nutrient availability or osmotic stress). This opens new opportunities to explore cell wall mechanosensitivity and its role in adapting tip growth to environmental signals. Significance StatementPlant growth relies on their ability to anchor roots in soil and maximize nutrient uptake. This process partly depends on root hairs. These long tubular extensions develop from the root surface, exhibiting a highly directional growth process --tip growth. Understanding how root hair growth adapts to soil mechanics is crucial, especially with climate change and soil hardening. We present a novel, non-invasive technique to probe the mechanics of root hair walls--key to their growth. By applying a feedback-controlled force, we can investigate the effect of mechanical resistance on root hair growth while preventing buckling, thus accessing elusive cell wall features. This method holds broader significance, as tip-driven growth is also used by fungi and yeasts to colonize their environments.

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BibTeXRIS

Alline, T., Cascaro, L., Durand-Smet, P., Couturier, E., Pereira, D., Asnacios, A.. 2025-10-10. Single Root hair growth under constant force: insights into wall mechanics. https://doi.org/10.1101/2025.10.09.681433

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