Rapid expansion of podoplanin-positive fibroblasts following radiation limits the anti-tumour CD8+ T-cell response to radiotherapy.

Wilkins, A. C.; Makris, S.; Green, M.; Hooper, S.; Mikolajczak, A.; Giangreco, G.; Allanki, S.; Nye, E.; Connick, E.; Mitter, R.; Cooper, S.; Durie, E.; Pedersen, M.; Burley, A.; O'Fee, K.; O'Leary, B.; Shewring, D.; Ramsden, Z.; Melcher, A.; Harrington, K.; Somaiah, N.; Acton, S. E.; Sahai, E.

doi:10.1101/2025.10.07.680949

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bioRxiv · 10.1101/2025.10.07.680949

Rapid expansion of podoplanin-positive fibroblasts following radiation limits the anti-tumour CD8+ T-cell response to radiotherapy.

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Abstract

Radiotherapy is known to cause changes in the tumour stroma which can undermine treatment efficacy. Our understanding of this process has historically centred around effects driven by Transforming Growth Factor-beta (TGF-{beta}) and alpha-smooth muscle actin (-SMA)+ fibroblasts. Here, we identified a rapid expansion of podoplanin (PDPN)+ fibroblasts following radiotherapy in breast, head and neck and melanoma tumours. This fibrosis was not dependent on TGF-{beta}, but was downstream of a radiotherapy-induced adaptive immune response. CD8+ T-cells entering the tumour after radiation were sequestered at the interface between residual tumour cells and PDPN+ fibroblasts and failed to enter the tumour core. Genetic deletion of PDPN in fibroblasts impacted their cytoskeleton and ability to organise extracellular matrix. This was associated with increased CD8+ T-cell entry and spontaneous tumour regression. Overall, we identify a mechanism whereby PDPN+ fibrosis limits immune-mediated radiation cell kill and demonstrate that disruption of PDPN signalling favours tumour control. SignificanceIn this study we show that rapid podoplanin (PDPN)+ fibroblast expansion following radiotherapy limits immune-mediated radiation cell kill. Targeting PDPN and associated downstream signalling improves tumour control and is a promising strategy in combination with radiotherapy. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=175 SRC="FIGDIR/small/680949v1_ufig1.gif" ALT="Figure 1"> View larger version (61K): org.highwire.dtl.DTLVardef@1711bb0org.highwire.dtl.DTLVardef@d0c3a1org.highwire.dtl.DTLVardef@1db88adorg.highwire.dtl.DTLVardef@1ea3ab6_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Wilkins, A. C., Makris, S., Green, M., Hooper, S., Mikolajczak, A., Giangreco, G., Allanki, S., Nye, E., Connick, E., Mitter, R., Cooper, S., Durie, E., Pedersen, M., Burley, A., O'Fee, K., O'Leary, B., Shewring, D., Ramsden, Z., Melcher, A., Harrington, K., Somaiah, N., Acton, S. E., Sahai, E.. 2025-10-07. Rapid expansion of podoplanin-positive fibroblasts following radiation limits the anti-tumour CD8+ T-cell response to radiotherapy.. https://doi.org/10.1101/2025.10.07.680949

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