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bioRxiv · 10.1101/2025.10.06.680743

Typical development of the human fetal subplate: regional heterogeneity, growth, and asymmetry assessed by in vivo T2-weighted MRI

Gondova, A.·Zhang, J.·You, S.·Jeong, S.·Candela-Leal, M. O.·Rollins, C. K.·Grant, P. E.·Yun, H. J.·Im, K.
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Abstract

The subplate (SP) is a transient fetal brain compartment supporting neuronal migration, axonal ingrowth, and early cortical activity, yet the dynamics of its regional development remain poorly understood in vivo. Using T2-weighted fetal MRI of 68 typically developing fetuses (22-32 weeks gestational age, GA), we developed a semi-automated pipeline to quantify regional SP morphology (thickness, surface area, and volume). SP characteristics scaled strongly with GA and residual brain volume and showed marked regional differences. After correcting for geometric confounds, regional variation of SP thickness persisted, with highest values in parietal and perisylvian regions, suggesting that SP thickness may serve as a sensitive marker of intrinsic developmental differences. Between late 2nd and early 3rd trimester, mean SP thickness increased by 39.2% with large variation across regions ({+/-}11.0 SD), whereas surface area growth was more uniform (64.3% {+/-}0.7 SD). Continuous growth trajectories clustered into distinct spatiotemporal profiles: early-developing regions (e.g., pericentral and medial occipital cortices) contrasted with later-developing regions (prefrontal, temporal, and parietal cortices). These patterns partially recapitulate primary-to-association, medial-to-lateral, and posterior-to-anterior maturational hierarchies, pointing to organized developmental program. SP development also showed region-specific hemispheric asymmetries, including leftward thickness and volume asymmetry in superior temporal and precentral gyri. Some asymmetries amplified, others attenuated or reversed with age, suggesting both transient states and potential precursors of postnatal lateralization. Together, these findings provide a framework for regional SP quantification and position SP morphology, particularly thickness, as a promising early biomarker that might link fetal SP changes to subsequent cortical development and neurodevelopmental outcomes. Significance StatementThe subplate (SP) is a transient fetal brain compartment that provides an early foundation for downstream cerebral development. Using fetal MRI, we show that SP morphology is regionally heterogeneous and that its growth follows distinct regional trajectories. Principal spatiotemporal patterns differentiate early-from later-developing regions and partially recapitulate sensory-to-association hierarchies of cortical maturation. SP also exhibits dynamic hemispheric asymmetries as early as the second trimester, with some amplifying as potential precursors of postnatal lateralization, and others reversing with age, emphasizing the importance of evaluating developmental trajectories rather than single time points. This framework positions SP morphology as an accessible early marker of fetal brain organization with potential relevance for later neurodevelopment.

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Gondova, A., Zhang, J., You, S., Jeong, S., Candela-Leal, M. O., Rollins, C. K., Grant, P. E., Yun, H. J., Im, K.. 2025-10-07. Typical development of the human fetal subplate: regional heterogeneity, growth, and asymmetry assessed by in vivo T2-weighted MRI. https://doi.org/10.1101/2025.10.06.680743

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