Isolation and genomic analysis of secondary metabolism in cave Actinomycetota

Bachmann, I. O.; Bachmann, B. O.

doi:10.1101/2025.10.06.680698

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bioRxiv · 10.1101/2025.10.06.680698

Isolation and genomic analysis of secondary metabolism in cave Actinomycetota

Bachmann, I. O.·Bachmann, B. O.

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Abstract

We report the isolation and genomic sequencing of nine Actinomycetota obtained from both cave biofilms and trogloxenic Ceuthophilus (cave crickets). Strains were isolated from samples via actinomycetota-selective media and sequenced using nanopore sequencing. Provisional taxonomic inference of strains was performed by whole genome phylogenetic analysis, revealing a broad range of genera, with most strains clustering with known genera with digital DNA-DNA hybridization values less than 70%, and one strain not clustering with known genera. An analysis of secondary metabolism reveals the quantity and diversity of secondary metabolism in cave Actinomycetota.

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Bachmann, I. O., Bachmann, B. O.. 2025-10-07. Isolation and genomic analysis of secondary metabolism in cave Actinomycetota. https://doi.org/10.1101/2025.10.06.680698

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