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bioRxiv · 10.1101/2025.09.29.679197

The peptide LyeTx I mnΔK induces transcriptomic reprogramming in a novel Multidrug-resistant Acinetobacter baumannii

Oliveira, F. G. d. C.·Barros, K. d. O.·Vianei, D. d. O.·Martins, J. R.·Duarte, J. C.·de Laet Souza, D.·Mamede, I.·Moreira, R. G.·De Aguiar, R. S.·da Silva, F. A.·dos Santos, V. L.·Varani, A. M.·Batista, T. M.·Machado, C. R.·Rosa, C. A.·de Lima, M. E.·Franco, G. R.
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Abstract

Acinetobacter baumannii is a critical pathogen in healthcare-associated infections, and treatment is challenging due to the emergence of multidrug-resistant strains. Antimicrobial peptides, such as LyeTx I mn{Delta}K, a synthetic peptide derived of a toxin from the spider Lycosa erythrognatha, represent a promising alternative due to their broad-spectrum activity and synergistic potential with antibiotics like meropenem. This study aimed to compare the genomes of several A. baumannii strains, including a novel multidrug-resistant A. baumannii isolate (AC37), and to evaluate the antimicrobial effects of LyeTx I mn{Delta}K-alone and in combination with meropenem-through transcriptomic analysis. Genome assembly and annotation of AC37 revealed 31 antibiotic resistance genes, and phylogenetic analysis comprising 123 A. baumannii genomes, including the reference strain, identified three unique resistant genes in the AC37 strain. Mobilome analysis showed 13 genes associated with mobile genetic elements, including two of the unique genes, highlighting horizontal gene transfer events. Transcriptomic profiling revealed that treatment with LyeTx I mn{Delta}K peptide alone induced several differentially expressed genes, including two efflux pump operons. Additionally, pathways related to protein synthesis, export, and secretion were activated, indicating a broader cellular response to the peptide. The treatment with LyeTx I mn{Delta}K in combination with meropenem disrupted oxidative phosphorylation, further revealing the metabolic plasticity of the bacterial response to external stresses. This study characterizes a new A. baumannii isolate and provides new insights into the bacterial response to a potential novel therapeutic molecule.

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Oliveira, F. G. d. C., Barros, K. d. O., Vianei, D. d. O., Martins, J. R., Duarte, J. C., de Laet Souza, D., Mamede, I., Moreira, R. G., De Aguiar, R. S., da Silva, F. A., dos Santos, V. L., Varani, A. M., Batista, T. M., Machado, C. R., Rosa, C. A., de Lima, M. E., Franco, G. R.. 2025-09-30. The peptide LyeTx I mnΔK induces transcriptomic reprogramming in a novel Multidrug-resistant Acinetobacter baumannii. https://doi.org/10.1101/2025.09.29.679197

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