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bioRxiv · 10.1101/2025.09.19.677421

Efficient generation of epitope-targeted de novo antibodies with Germinal

Abstract

Obtaining novel antibodies against specific protein targets is a widely important yet experimentally laborious process. Meanwhile, computational methods for antibody design have been limited by low success rates that currently require resource-intensive screening. Here, we introduce Germinal, a broadly enabling generative pipeline that designs antibodies against specific epitopes with nanomolar binding affinities while requiring only low-n experimental testing. Our method co-optimizes antibody structure and sequence by integrating a structure predictor with an antibody-specific protein language model to perform de novo design of functional complementarity-determining regions (CDRs) onto a user-specified structural framework. When tested against four diverse protein targets, Germinal successfully designed functional antibodies across all targets and binder formats, testing only 43-101 designs for each antigen. Validated designs also exhibited robust expression in mammalian cells and high sequence and structural novelty. We provide open-source code and full computational and experimental protocols to facilitate wide adoption. Germinal represents a milestone in efficient, epitope-targeted de novo antibody design, with notable implications for the development of molecular tools and therapeutics.

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BibTeXRIS

Mille-Fragoso, L. S., Wang, J. N., Driscoll, C. L., Dai, H., Widatalla, T. M., Zhang, X., Hie, B. L., Gao, X. J.. 2025-09-24. Efficient generation of epitope-targeted de novo antibodies with Germinal. https://doi.org/10.1101/2025.09.19.677421

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