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bioRxiv · 10.1101/2025.09.17.676746

Changes in Urinary Proteome Post-Translational Modifications Following Short-Term Intake of Specific Magnesium, Calcium, Zinc, and Iron Supplements in Rats

Su, Y.·Shen, Z.·Gao, Y.
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Abstract

Magnesium, calcium, zinc, and iron are essential mineral elements indispensable for maintaining physiological functions in mammals. This study investigated changes in post-translational modifications (PTMs) of the urinary proteome in Sprague-Dawley (SD) rats of different ages following short-term intragastric administration of magnesium L-threonate (MgT), calcium gluconate, zinc gluconate, and polysaccharide-iron complex. The results revealed PTM alterations in the urinary proteins of rats in all mineral supplement groups, with the most pronounced changes observed in the polysaccharide-iron complex group. Notably, rats of different ages exhibited distinct responses to the same mineral supplement. The proteins carrying differential PTMs were involved in a wide range of biological functions, and several of them, including serotransferrin and beta-2-microglobulin, are closely associated with mineral metabolism. This study explores the functional changes in urinary proteins from a PTM perspective following short-term mineral supplementation in rats, providing a novel viewpoint for investigating the physiological functions of mineral elements.

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Su, Y., Shen, Z., Gao, Y.. 2025-09-20. Changes in Urinary Proteome Post-Translational Modifications Following Short-Term Intake of Specific Magnesium, Calcium, Zinc, and Iron Supplements in Rats. https://doi.org/10.1101/2025.09.17.676746

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