Regulation of epithelial tissue homeostasis by active trans-epithelial transport

Abstract

Epithelia are intricate tissues whose function is intimately linked to mechanics. While mechanobiology has primarily focused on factors such as cell-generated contractility and mechanical properties of extracellular matrix (ECM), a novel mechanobiological paradigm highlights the role of osmotic and mechanical pressures in shaping epithelial tissues. In our study, we developed an in vitro model of cell coated micro-sized hydrogel spheres (MHSs) which allows to decipher the interplay between cellular activities and tissue mechanics. Drastic, isotropic MHS compressions were observed once the epithelia reached confluence. Further studies revealed that the compression was a process independent of cell contractility but rather regulated by active transepithelial fluid flow. Compressive stresses of about 7 kPa are generated by such an active hydraulic mechanism. Tissue homeostasis is then maintained by a fine balance between cell proliferation and extrusion. Our findings demonstrate the critical role of fluid transport in generating mechanical forces within epithelial tissues. Supported by a theoretical mechano-hydraulic model, a mechanistic framework for understanding the intricate interplay between cellular processes and tissue mechanics was established. These results challenge traditional views of epithelial tissue mechanics, emphasizing the pivotal influence of osmotic and mechanical pressures in shaping tissues. We anticipate that this study will advance the understanding of epithelial tissue development, the maintenance of homeostasis, and the mechanisms underlying pathological conditions. Significance StatementEpithelial tissues are vital for many bodily functions, but their mechanics remain poorly understood. Our study uncovers a novel mechanism by which epithelial cells generate mechanical stress, not through traditional cell contractility, but by actively pumping ions and water across their membranes. Using a model of micro-sized hydrogel spheres (MHSs) coated with epithelial cells, we demonstrate that epithelial cells actively transport fluid across the tissue to compress the MHSs. This facilitates the establishment of tissue homeostasis, which is further maintained by a balanced cell proliferation and extrusion rate. Supported by a theoretical model, our findings highlight the overlooked role of fluid transport in tissue mechanics, offering new insights into how epithelial tissues develop, maintain stability, and respond to disease.