Allelic variation in MAL33 drives ecological adaptation of maltose metabolism in Saccharomyces eubayanus

Quintrel, P.; Munoz-Guzman, F.; Villarreal, P.; Pena, T. A.; Garate, N. I.; Munoz-Tapia, C.; Oporto, C. I.; CrandallCrandall, J. G.; Larrondo, L. F.; Hittinger, C.; Fischer, G.; Cubillos, F. A.

doi:10.1101/2025.09.15.676268

bioRxiv · 10.1101/2025.09.15.676268

Allelic variation in MAL33 drives ecological adaptation of maltose metabolism in Saccharomyces eubayanus

Quintrel, P.·Munoz-Guzman, F.·Villarreal, P.·Pena, T. A.·Garate, N. I.·Munoz-Tapia, C.·Oporto, C. I.·CrandallCrandall, J. G.·Larrondo, L. F.·Hittinger, C.·Fischer, G.·Cubillos, F. A.

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Abstract

Maltose is one of the most abundant sugars in brewers wort, and its efficient utilization is critical for successful fermentation. However, maltose consumption varies naturally among Saccharomyces eubayanus strains isolated from different host trees, such as Quercus and Nothofagus. To identify the genetic determinants underlying these phenotypic differences, we performed bulk segregant analysis (BSA) and quantitative trait loci (QTL) mapping using an F2 offspring derived from QC18 (Quercus-associated) and CL467.1 (Nothofagus-associated) strains. QTL mapping identified two significant genomic regions on subtelomeric loci of chromosomes V-R and XVI-L, each containing complete MAL loci composed of MAL32 (encoding maltase), MAL31 (transporter), and MAL33 (transcriptional activator) genes. Comparative polymorphism analyses identified mutations in MAL32 and MAL33 of QC18, including frameshift mutations resulting in premature stop codons. Functional validation demonstrated that the heterologous expression of MAL33ChrV from CL467.1 fully restored maltose utilization in QC18, indicating the functional presence of MAL33 cis-regulatory sequences and MAL32 and MAL31 genes in QC18. While structural protein predictions identified truncation and impaired functionality in the maltose-responsive activation domain of Mal33p from QC18, overexpression of QC18s own MAL33ChrV allele also improved maltose metabolism, suggesting dosage-dependent transcriptional limitations rather than complete functional loss. These results indicate that allelic variations in the maltose-responsive activation domain of Mal33p lead to differences in maltose consumption between strains. We hypothesized that reduced maltose metabolism in QC18 is an adaptive response to the distinct sugar composition in Quercus robur bark, contrasting with the starch-rich environment of Nothofagus pumilio. These findings highlight subtelomeric MAL gene diversity as a reservoir of evolutionary plasticity, representing a key evolutionary mechanism that influences maltose adaptation among natural Saccharomyces isolates.

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Quintrel, P., Munoz-Guzman, F., Villarreal, P., Pena, T. A., Garate, N. I., Munoz-Tapia, C., Oporto, C. I., CrandallCrandall, J. G., Larrondo, L. F., Hittinger, C., Fischer, G., Cubillos, F. A.. 2025-09-19. Allelic variation in MAL33 drives ecological adaptation of maltose metabolism in Saccharomyces eubayanus. https://doi.org/10.1101/2025.09.15.676268

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