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bioRxiv · 10.1101/2025.09.12.675811

Micro and nanoplastic inhalation during pregnancy elicits uterine endothelial dysfunction in Sprague Dawley rats by impeding NO signaling

Abstract

Micro and nanoplastic (MNP) detection in human tissues demonstrates that exposure at any life stage is inevitable. We have previously demonstrated that pulmonary exposure to this emerging environmental contaminant impairs endothelial function in the uterine vasculature of nonpregnant and pregnant rats. However, neither the mechanism of this dysfunction nor the role of the endothelial-derived vasodilator, nitric oxide (NO), have been interrogated. Therefore, we assessed uterine macro- and microvascular reactivity in Sprague Dawley rats to determine the mechanistic role of NO signaling in endothelial dysfunction after repeated (gestational day 5-19) MNP inhalation during pregnancy. Results identified that MNP exposure reduced fetal growth and impaired endothelial-dependent dilation in the uterine microcirculation, which control placental perfusion and resource availability to the fetus. Levels of activated endothelial nitric oxide synthase (eNOS), phosphorylated on Ser1176, were substantially decreased (<50%) in uterine vessels from exposed rats. This suggests MNP inhalation limited NO production and bioavailability. Endothelial function was partially restored by supplementation of arterial segments with the eNOS cofactor tetrahydrobiopterin (BH4), demonstrating that exposed vessels were BH4-deficient. Partial restoration was also achieved by incubation with the reducing agent, DTT, suggesting that exposed vessels contained physiologically relevant levels of reactive oxygen and nitrogen species. Increased 3-nitrotyrosine residues and decreased thioredoxin protein expression further suggest MNP fosters nitrosative and oxidative stress in the uterine vasculature, impairing eNOS and endothelial-dependent dilation. These findings implicate eNOS uncoupling as a mechanistic basis for the vascular toxicity of MNPs and the adverse impact of MNPs on fetal development. NEW & NOTEWORTHYThis study reveals that repeated micro and nanoplastic (MNP) inhalation throughout gestation blunts endothelial-dependent dilation in the uterine microcirculation, promoting fetal growth restriction. Exposure impaired endothelial nitric oxide signaling through deactivating endothelial nitric oxide synthase (eNOS), reducing the availability of the eNOS cofactor tetrahydrobiopterin and producing a nitrosative and oxidative environment in uterine vascular tissue. These novel findings highlight the eNOS uncoupling as a key mechanism behind the fetal growth restriction induced by MNP.

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BibTeXRIS

Cary, C. M., Moore, T. L., Gow, A. J., Stapleton, P. A.. 2025-09-17. Micro and nanoplastic inhalation during pregnancy elicits uterine endothelial dysfunction in Sprague Dawley rats by impeding NO signaling. https://doi.org/10.1101/2025.09.12.675811

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