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bioRxiv · 10.1101/2025.09.08.675014

Single-cell multiomics reveals archetypal regulatory programs shared across CD4 and CD8 T cell subsets in viral infection

Abstract

T cells protect against pathogens and tumors and can differentiate into various functionally distinct subsets. While each subset exhibits a characteristic epigenomic and transcriptional profile, essential immune functions such as self-renewal, expansion, cytokine production, and cytotoxicity are common to multiple subsets and therefore must be programmed via shared regulatory mechanisms. To uncover these regulatory "archetypes", we integrated a new single-cell multiomic (scATAC+RNA-seq) dataset for CD4 and CD8 T cells responding to acute and chronic viral infection with a comprehensive compendium of bulk ATAC-seq data. Our analysis revealed that T cell identity is governed by a modular architecture, where distinct transcription factors drive the reuse of regulatory archetypes across T cell subsets and lineages. Notably, translationally important progenitor Tcf1+ CD8 T cells, critical for sustaining CD8 T cell responses and present in both acute and chronic infection, exhibited a composite regulatory state combining a CD8 T cell exhaustion program and, unexpectedly, a follicular helper CD4 T cell program. In sum, this resource will aid mechanistic dissection of adaptive immunity and immunotherapy design, and the framework will be broadly applicable across biological systems. Highlights- Single-cell multiomics and ATAC-seq atlas reveal T cell regulatory archetypes - A unified progenitor Tcf1+ CD8 T cell population is shared by acute and chronic infection - Progenitor CD8 T cell identity combines CD8 exhaustion and CD4 Tfh regulatory programs - A Tfh-like differentiation gradient underlies heterogeneity in progenitor CD8 T cells

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Walker, S. K., van der Veeken, J., Rudensky, A., Pritykin, Y.. 2025-09-13. Single-cell multiomics reveals archetypal regulatory programs shared across CD4 and CD8 T cell subsets in viral infection. https://doi.org/10.1101/2025.09.08.675014

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