Using gas exchange measurements to monitor growth, energy expenditure, and body composition of black soldier fly larvae (Hermetia illucens) on diets varying in protein-to-carbohydrate ratio

Schon, M. L.; Jensen, K.; Schow-Madsen, M.; Gold, M.; Mathys, A.; Schou, T. M.; Berggreen, I. E.; Norgaard, J. V.; Overgaard, J.

doi:10.1101/2025.09.08.674872

bioRxiv · 10.1101/2025.09.08.674872

Using gas exchange measurements to monitor growth, energy expenditure, and body composition of black soldier fly larvae (Hermetia illucens) on diets varying in protein-to-carbohydrate ratio

Schon, M. L.·Jensen, K.·Schow-Madsen, M.·Gold, M.·Mathys, A.·Schou, T. M.·Berggreen, I. E.·Norgaard, J. V.·Overgaard, J.

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Abstract

Optimizing black soldier fly larvae (BSFL) production requires a better understanding of how diet composition shapes growth, nutrient allocation, and metabolic efficiency. This study investigated whether respiratory measurements could serve as predictive, non-invasive tools for BSFL performance on diets differing in protein-to-carbohydrate (P:C) ratio. Larvae were reared for seven days on four experimental diets, with growth, survival, gas exchange (O2 consumption, CO2 production, respiratory exchange ratio (RER)), and body composition (crude protein and lipid contents) measured across seven replicates per treatment. O2 consumption correlated strongly with final larval biomass, while RER was closely associated with protein and lipid deposition. Larvae on low-protein, high-carbohydrate diets accumulated proportionally more lipid, whereas protein-rich diets increased crude protein content but required higher energetic investment. This demonstrates that lipid-rich larvae grew more efficient in energetic terms per unit accumulated biomass than protein-rich larvae. Across diets, gas exchange variables reliably reflected growth dynamics, metabolic activity, and nutrient assimilation. Integrating respirometry into rearing systems could enable real-time monitoring of insect biomass yield and nutrient composition.

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Schon, M. L., Jensen, K., Schow-Madsen, M., Gold, M., Mathys, A., Schou, T. M., Berggreen, I. E., Norgaard, J. V., Overgaard, J.. 2025-09-13. Using gas exchange measurements to monitor growth, energy expenditure, and body composition of black soldier fly larvae (Hermetia illucens) on diets varying in protein-to-carbohydrate ratio. https://doi.org/10.1101/2025.09.08.674872

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