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bioRxiv · 10.1101/2025.09.02.673790

Simulation of evoked responses to transcranial magnetic stimulation using a multiscale cortical circuit model

Zhao, Z.·Aberra, A. S.·Opitz, A.
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Abstract

BackgroundTranscranial magnetic stimulation (TMS) is a widely used non-invasive brain stimulation technique, but the neural circuits activated by TMS remain poorly understood. Previous modeling approaches have been limited to either simplified point-neuron networks or isolated single-cell models that lack synaptic connectivity. ObjectiveTo develop and validate a multiscale cortical circuit model that integrates morphologically-realistic neurons with accurate TMS-induced electric field distributions and to investigate mechanisms underlying cortical responses to stimulation. MethodsWe constructed a network model of a cortical column comprising 10,000 biophysically realistic neurons (excitatory pyramidal cells and inhibitory interneurons) across layers 2/3, 5, and 6 with over 10 million synaptic connections. The model incorporated thalamic and non-specific corticocortical inputs to generate physiological firing rates. TMS-induced electric fields were calculated using finite element modeling and coupled to individual neurons through the extracellular mechanism. We validated model predictions against experimental recordings of TMS-evoked local field potentials (LFPs) and multiunit activity. ResultsThe model reproduced key experimental observations including the dose-dependent N50 LFP component and multiphasic multi-unit responses, consisting of an (excitatory) increase followed by a (inhibitory) decrease in firing rates. The early excitatory response exhibited dual-peak dynamics reflecting distinct contributions from directly and indirectly activated neuronal populations, and the subsequent inhibitory phase reflected activation of feedback GABAergic circuits through both GABAA and GABAB conductances. Spatial analysis across 30 cortical columns distributed across the precentral gyrus revealed orientation-dependent evoked responses. ConclusionThis validated multiscale model provides mechanistic insights into TMS-evoked cortical dynamics, demonstrating how direct neuronal activation cascades through synaptic networks to generate characteristic population responses. The framework establishes a computational platform for optimizing stimulation protocols in research and clinical applications. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=124 SRC="FIGDIR/small/673790v1_ufig1.gif" ALT="Figure 1"> View larger version (37K): org.highwire.dtl.DTLVardef@1028145org.highwire.dtl.DTLVardef@1751714org.highwire.dtl.DTLVardef@3e2c06org.highwire.dtl.DTLVardef@1bc756e_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Zhao, Z., Aberra, A. S., Opitz, A.. 2025-09-07. Simulation of evoked responses to transcranial magnetic stimulation using a multiscale cortical circuit model. https://doi.org/10.1101/2025.09.02.673790

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