Characterizing representational shaping of individual motor and object representations after sequence learning

Abstract

Learning temporal regularities between pairs of events has been shown to shape neural representations in the medial temporal lobe, but it remains unclear whether representational changes generalize across memory domains. Here we used fMRI and multivoxel pattern similarity analyses to examine representational shaping as a consequence of motor and object sequence learning. Across two sessions, participants incidentally learned a fixed four-element sequence of finger movements or visual objects. We compared the pattern of blood oxygen level-dependent activity evoked by each object and finger movement before and after sequence learning. Analyses were performed on bilateral ROIs, including primary motor cortex (M1), lateral occipital complex (LOC), premotor cortex (PMC), striatum (STR) and the hippocampus (HC). Behaviorally, participants successfully learned the sequence in both domains. At the neural level, motor representations became more differentiated with learning in M1, PMC, STR, and HC, but not in LOC. This global differentiation was not specific to the sequence condition and was also observed after repeated co-occurrence of movements in a pseudorandom order. Critically, the HC differentiated between motor representations in the learned sequence in a unidirectional predictive manner whereby movements became more differentiated from the preceding than from the following element in the sequence. In contrast, object representations remained stable across sequence repetitions in all brain regions, even though participants successfully learned their temporal order.