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bioRxiv · 10.1101/2025.08.25.672205

Early Metabolic Alterations in Cerebrospinal Fluid Fatty Acid Profiles Linked to Cognitive Decline and All-Cause Dementia

Ayembilla, J. A.·Wadop, Y. N.·Bernal, R.·Ayele, B. A.·Sargurupremraj, M.·Jian, X.·Njamnshi, A. K.·Fongang, B.·Fonteh, A. N.
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Abstract

BackgroundWhile All-cause dementia (ACD) may often be characterized by the abnormal deposition of extracellular {beta}-amyloid (A{beta}) in the brain cortex and hyperphosphorylated tau (p-tau) as neurofibrillary tangles intracellularly, there is a need to identify early metabolic changes that may accompany these pathological changes. ObjectiveThis study evaluated the predictive value of fatty acids in cerebrospinal fluid (CSF) fractions in differentiating cognitively unimpaired (CU), mild cognitive impairment (MCI), and ACD participants. MethodsCSF fatty acid profiles were analyzed from CU (n=68), MCI (n=38), and ACD (n=37) individuals aged 77.3 {+/-} 7.7 years, sourced from the Huntington Medical Research Institutes (HMRI). Multivariable binary logistic regression identified the most effective CSF fatty acid biomarkers for distinguishing CU, MCI, and ACD groups. The top-performing CSF fatty acid biomarkers were combined with A{beta}42, tau, and the A{beta}42/tau ratio to evaluate their collective diagnostic performance. The model was adjusted for covariates, including age, sex, smoking status, hypertension, diabetes, and APOE genotype. Receiver operating characteristic (ROC) curves were generated for the top 10 CSF fatty acids ranked by area under the curve (AUC), sensitivity, and specificity, and their significance was assessed using the DeLong test. ResultsThe top 10 fatty acids in CSF fractions demonstrated superior discrimination between CU individuals and those with MCI compared to traditional markers such as A{beta}42, tau, and the A{beta}42/tau ratio. Furthermore, incorporating a panel of these fatty acid biomarkers alongside A{beta}42/tau significantly improved diagnostic accuracy. Age, sex, smoking, hypertension, APOE genotype, and diabetes did not significantly influence the models performance. ConclusionThis study suggests that changes in fatty acid metabolism occur in early ACD pathology. Thus, strategies that regulate fatty acid metabolism may prevent cognitive decline in an older population. HighlightsO_LIThis study revealed that CSF fractions fatty acids have a better discriminatory power for CU from MCI than CSF A{beta}42, tau, and A{beta}42/tau ratio. C_LIO_LIA panel of CSF fraction fatty acids combined with A{beta}42/tau remarkably improved its diagnostic performance for differential diagnosis of CU, MCI, and ACD. C_LIO_LIClinical evaluation of these fatty acids will strengthen the detection of early cognitive impairment. A prospective large cohort multicenter study of the diagnostic utility of CSF fractions fatty acids will provide robust evidence before extensive clinical usage of these fatty acids. C_LI

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Ayembilla, J. A., Wadop, Y. N., Bernal, R., Ayele, B. A., Sargurupremraj, M., Jian, X., Njamnshi, A. K., Fongang, B., Fonteh, A. N.. 2025-08-30. Early Metabolic Alterations in Cerebrospinal Fluid Fatty Acid Profiles Linked to Cognitive Decline and All-Cause Dementia. https://doi.org/10.1101/2025.08.25.672205

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