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bioRxiv · 10.1101/2025.08.21.671622

A Flexible Quadruple-Stranded Helicate Demonstrates a Strong Binding Preference for DNA Three-Way Junctions by Induced-Fit

Williams, H. D.·Dettmer, S. J.·Bajpai, S.·Hannon, M. J.
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Abstract

Nucleic acid junctions are key to many biological functions from recombination and repair to viral NA insertion, and are an attractive, functional biomolecular target. We describe a quadruple-stranded di-platinum helicate that binds both three-way (3WJ) and four-way DNA junctions (4WJ). This allows us to probe the relative importance of size and shape in junction-binder design. Despite the helicates tetragonal symmetry/shape being compatible with the 4WJ, microscale thermophoresis (MST), isothermal calorimetry (ITC) and gel electrophoresis competition experiments demonstrate that this metallo-supramolecule displays a stronger affinity for 3WJs (Kd = 12 nM) than for 4WJs (Kd > 4 {micro}M) and other DNA structures. The experimental findings are supported by molecular dynamics simulations which reveal the critical role of size. Whilst the open form of the 4WJ is promoted when the helicate is in the cavity, the helicates small size means it is unable to maintain {pi} contacts with all four junction base-pairs simultaneously. Although the helicate is slightly too large for the smaller 3WJ cavity, simulations and experiments show that it can open up the cavity (increasing the junctions hydrodynamic radius) by disrupting a base-pair. The flexible helicate also responds to the cavity upon binding by favouring one enantiomer and allowing the helicate to adopt a stable final structure inside the 3WJ that is an induced-fit of the two dynamic structures (supramolecule and DNA). This contrasts with previous lock-and-key examples of junction recognition and opens up new possibilities for how to design DNA and RNA junction-binding compounds. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=72 SRC="FIGDIR/small/671622v1_ufig1.gif" ALT="Figure 1"> View larger version (19K): org.highwire.dtl.DTLVardef@175cabborg.highwire.dtl.DTLVardef@50f114org.highwire.dtl.DTLVardef@372e1org.highwire.dtl.DTLVardef@6b2960_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Williams, H. D., Dettmer, S. J., Bajpai, S., Hannon, M. J.. 2025-08-25. A Flexible Quadruple-Stranded Helicate Demonstrates a Strong Binding Preference for DNA Three-Way Junctions by Induced-Fit. https://doi.org/10.1101/2025.08.21.671622

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