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bioRxiv · 10.1101/2025.08.13.667888

Uncovering an unconventional JAK1/2-STAT3 branch in macrophage IFNγ signaling

Abstract

Interferon-{gamma} (IFN{gamma}) is a key cytokine in immune activation, especially anti-viral responses and driver of macrophage activation. It classically signals via JAK1/2-mediated STAT1 homodimers. Here, we identify an alternative, non-canonical signaling component in which IFN{gamma} simultaneously also activates STAT3. Our results show that IFN{gamma} activates STAT3 rapidly and directly through JAK1 and JAK2. We provide the first evidence that STAT3 can form heterodimers with STAT1 in this context and demonstrate that STAT3 is co-recruited to a subset of IFN{gamma}-induced, STAT1-bound regulatory elements. While IFN{gamma} directly activates STAT3, our results reveal that its contribution to gene regulation is limited, indicating that STAT1 easily substitutes the STAT1-STAT3 heterodimer for STAT1 homodimers when STAT3 is absent. These findings uncover STAT3 as a new unconventional player in macrophage IFN{gamma} signaling, underscoring the complex and context-dependent nature of cytokine signaling networks.

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BibTeXRIS

Botman, M., Huisman, J. M. A., Drakaki, A., Maas, S. L., Witalisz-Siepracka, A., Stoiber, D., van der Vorst, E. P. C., Siebeler, R., Hoeksema, M. A.. 2025-08-15. Uncovering an unconventional JAK1/2-STAT3 branch in macrophage IFNγ signaling. https://doi.org/10.1101/2025.08.13.667888

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