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bioRxiv · 10.1101/2025.08.12.670003

Genetically encoded tool for manipulation of ATP/ADP ratio in human cells

Ekvik, A. E.·Kober, M. M.·Titov, D. V.
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Abstract

The ability of cells to power energy-demanding processes depends on maintaining the ATP hydrolysis reaction a billion-fold away from equilibrium. Cells respond to changes in energy state by sensing changes in ATP, ADP, AMP, and inorganic phosphate. A key barrier to a better understanding of the maintenance of energy homeostasis is a lack of tools for direct manipulation of energy state in living cells. Here, we report the development of ATPGobble-a genetically encoded tool for controlling cellular ATP hydrolysis rate. We validated ATPGobble by showing that it doubles the energy demand, decreases [ATP]/[ADP] and [ATP]/[AMP] ratios, and activates AMPK activity in human cells. We then used ATPGobble to systematically characterize the proteome and phosphoproteome changes caused by direct manipulation of the energy state. Our results establish ATPGobble as a powerful approach for dissecting the regulatory roles of energy state in human cells, opening new opportunities to study how cellular energy state governs physiology, stress responses, and disease processes.

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Ekvik, A. E., Kober, M. M., Titov, D. V.. 2025-08-15. Genetically encoded tool for manipulation of ATP/ADP ratio in human cells. https://doi.org/10.1101/2025.08.12.670003

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